Sullivan's reaction for the quantitative determination of cysteine and cystine

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Flow Injection Spectrofluorimetric Determination of Cystine and Cysteine

Um procedimento relativamente simples e sensível com detecção espectrofluorimétrica foi desenvolvido para a determinação de cistina e cisteína por sistema de injeção em fluxo com determinação seqüencial. Esse método é fundamentado na redução de Tl(III) com cisteína em meio ácido, produzindo o reagente fluorescente TlCl 3 2(λ ex = 227 nm, λ em = 419 nm). Antes da injeção, a solução da amostra fo...

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The chromatographic determination of cystine and cysteine residues in proteins as s-beta-(4-pyridylethyl)cysteine.

Disullide bonds in proteins and in seed meals were reduced with /3-mercaptoethanol to sulfhydryl groups. The generated sulfhydryl groups were then alkylated by treatment with 4-vinylpyridine. The cysteine residues were thus derivatized to S-(4-pyridylethyl)-L-cysteine residues. The cysteine derivative is stable to acid hydrolysis and on amino acid analysis by ion exchange elutes just before arg...

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On the Reversible Reaction of Cyanate with Sulfhydryl Groups and the Determination of Nh2-terminal Cysteine and Cystine in Proteins.

The present paper is a continuation of studies on the reaction of cyanate with amino acids, peptides, and proteins (1). In the earlier report, emphasis was placed on the speed of the reaction of cyanate with the sulfhydryl groups of cysteine, glutathione, and urea-denatured P-lactoglobulin. The work to be presented in this communication is concerned primarily with a study of the cyanate-sulfhyd...

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On the Reversible Reaction of Cyanate with Sulfhydryl Groups and the Determination of NH,-terminal Cysteine and Cystine in Proteins*

The present paper is a continuation of studies on the reaction of cyanate with amino acids, peptides, and proteins (1). In the earlier report, emphasis was placed on the speed of the reaction of cyanate with the sulfhydryl groups of cysteine, glutathione, and urea-denatured P-lactoglobulin. The work to be presented in this communication is concerned primarily with a study of the cyanate-sulfhyd...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1933

ISSN: 0306-3283

DOI: 10.1042/bj0270668